We already tried dilutions in 0.9%Nacl and 2.9% sodium citrate, followed by incubation for 20min at 37°C
Sava Micic
As a student in Bachelor degree program in computer science field we have a project in course of "Language Theory", our project related with Natural Language Processing: - First phase talks...
25 April 2024 6,961 3 View
There are many commercial mixtures and nutritional supplements that claim to raise testosterone levels in men, and they are based on several types of natural honey. Could these nutritional...
20 September 2023 3,003 2 View
Increasing the chlorophyll content can induce an increase in grain yield in durum wheat?
06 May 2022 7,869 6 View
Abdelkrim Boujraf looks for data scientists who train Machine Learning models and can invest
31 October 2021 6,258 7 View
For a statistical study easy to do, solid and reliable in ecology, use you R-SPSS-SAS-EXCELSTAT or other software?
03 October 2020 8,600 5 View
Can I synthesize the silicon dioxide nanoparticles from desert sands? What is the easiest and least expensive way to achieve this if it is possible?
02 August 2020 2,356 5 View
How can I produce bioethanol that is not contaminated with methanol?
27 March 2020 4,353 6 View
There are different standards for each factory and for each country for the heavy metals concentration ,but i want to know which one is useful especially in publication case.
05 February 2020 946 5 View
I am looking for a reliable method for dating azoic sediments (conglomerates, sandstones and mudstones). they are triassic-Jurassic red sediments separated by the CAMP.
30 December 2018 8,829 4 View
I used befor lime (Ca(OH)2), caustic soda (NaOH) and sodium carbonate (Na2CO3) individually , and i need other reactif to make a comparison study to know the best reactif between them .
21 July 2018 966 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View