I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to achieve the sensitivity diagram of a sensor in CST?

I wanna design a sensor in CST 2021 for the first time and I don't know how to achieve the sensitivity diagram of the sensor in CST. Could you plz help me to do that? I appreciate your response...

26 January 2021 6,864 1 View

What kinds of potential genes could be expressed in frogs exposed to chytrid fungus in different environmental conditions ?

I need an applicable molecular biomarker like expressing the genes in frogs who expose to chytrid fungus in different environmental stressors. Indeed, what kinds of genes I should notice in frog's...

25 January 2021 7,718 2 View

How to select the most non-correlated samples within a dateset?

Dear researchers How to select the most non-correlated samples within a dateset? Considering that the samples are time-series of length l within m different time-series. To more clarify the...

13 January 2021 741 6 View

DSC test- Glass transition temperature of the electrospun PA66 nanofibers?

Hello Everyone, I did several DSC tests on the electrospun PA66 nanofibers to obtain the glass transition temperature. According to the material datasheet, the Tg point of PA66, as cast, is around...

13 December 2020 1,506 2 View

How to create a sag and swell detection in the DVR applications?

I have created a three-phase cascaded h-bridge inverter at Simulink with the SHE-PWM technique, and I want to test it in the DVR applications. How can I design a controller for that in the DVR...

13 December 2020 735 3 View

Degenerated semiconductor (Burstein Moss effect) with unchanged band-gap?

Is it possible to produce a degenerate semiconductor using a dopant agent that results in increasing the electrical conductivity, but the optical band-gap remains unchanged? In such a situation...

11 December 2020 8,338 13 View

Which statistic software can give me a relationship for multivariate function?

Hi friend I have some data and they influence on each others and I draw their graph in excel but excel can't give me a relationship for multivariate function. the regression of these data in excel...

29 November 2020 9,371 9 View

A paper may be rejected because of several reasons?

In my case, I have received rejection after seven months from submission to journal without reason or editor comments (decline). Moreover, without being reviewed by an expert scientist reviewer,...

29 October 2020 5,437 4 View

Do scalar-tensor-vector gravity theories violate the Bekenstein-Hawking area law to compute the entropy of black holes?

I am looking for the thermodynamics of the cosmological black hole in scalar-tensor-vector gravity theories. I know how should I write and find the thermodynamical quantities and equations for...

26 September 2020 6,383 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

Gromacs force field and water model recommended for protein hydrophobic study?

Dear All, I received a comment from reviewer about asking me selection of force field and water model. I choose OPLS-AAL and 3-point water model. They mentioned the combination of OPLS-AAL and SPC...

13 February 2021 1,364 3 View

In what type of container can we put a 200 degree Celsius poly alkylene glycol? what should be the material of the container?

I am having a 200-degree Celcius heated poly alkylene glycol now I want to know in which type of container should I put that heated glycol or what should be the material of the container?

08 February 2021 6,819 1 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How can you make a PEDOT:PSS film using the doctor blade technique?

I have been trying to make an even PEDOT: PSS (PH1000) film with the doctor blade technique (tape casting) with varying results. I have been using a silicon substrate that's treated with piranha...

01 February 2021 8,506 5 View

How to perform hydrogen underpotential in hydrophobic material?

I have a working electrode composed of deposited graphene material (confirmed from Raman).The graphene is porous and has deposited Pd nanoparticles. I am trying to perform hydrogen underpotential...

01 February 2021 3,199 1 View

What could be possible reason of higher Drug Concentration of Liposomal formulation stability samples over time?

Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...

25 January 2021 7,752 4 View