How can the government regulate the mini-bus operations which is the dominant transport mode in Southern Africa region?

08 April 2020 0 6K Report

Southern Africa regional passenger transport, is mostly mini-buses and the rest,

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Why is absorbance measured at 540 nm instead of at 280 nm when quantifying proteins in Lowry and Buiret methods?

Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...

16 December 2020 1,010 3 View

Can you help with method how to compare two emulsions for stability for FAT Oil and grease emulsification?

I'm trying to compare several different surfactant efficiencies for Swage Fato Oil and Grease emulsification into water. I don't think that methods for fat determination in food for example...

06 October 2020 4,941 1 View

What is the importance of need for achievement as a character of entrepreneurs in understanding entrepreneurship?

The three personality characteristics that are documented in almost all entrepreneurship documented information are: i. Need for Achievement ii. Internal Locus of Control iii. Risk-taking Why in...

17 May 2020 9,849 10 View

A host of papers related to wastewater treatment are published every year, what percentage of these papers are implemented at large scale?

Are the researchers doing enough to assist to the betterment of our society?

03 January 2020 5,554 2 View

No band after SDS PAGE, so what can i do?

Good afternoon. I have a problem detecting proteins on my gel after SDS PAGE. After extracting the protein, i quantified using the Lowry's method and had concentrations ranging between 2 to...

29 August 2016 5,994 4 View

What can I do to have pcr products with A-T rich sequences using genomic DNA as template?

I have six constructs with the following G-C contents: 29.6%, 23.2%, 27.2%, 24.4%, 27% and 28%. Due to the low G-C content of genes of interest, the Tm of my primers (see attached file) are low;...

03 March 2015 9,011 9 View

If kept antibody in serum sample at 4 degrees C, necessary supplements what solution for stable?

Can water plus 0.02% merthiolate ?

12 March 2012 7,599 1 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Does Location Map of Informal Settlements in South Africa exist?

I urgently need a map that shows the point locations of informal settlements in South Africa. I am currently working on a study that seeks to relate protests location to the location of informal...

02 February 2021 4,113 7 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

Kindly suggest sources for covid 19 data over Africa ?

Kindly suggest sources for covid 19 data for all countries of Africa included different provinces if available? Thanks in advance

17 January 2021 6,810 7 View

Johnson et al., 2006 Geology of South Africa?

I am wondering if there is a digital (PDF) version of this book available or if anyone is willing to share this work. It is no longer available from GSSA (Geo Soc. S. Afr) nor does my library hold...

13 January 2021 5,571 2 View

Fuzzy regulator applied to the asynchronous machine?

hello, in fact I encountered problems during the simulation of the fuzzy regulator applied to the asynchronous machine. I replaced the PI regulator of the vector control with the fuzzy regulator,...

12 January 2021 6,131 3 View

Is recycling a challenge to the governments of developing countries?

Is recycling a challenge to the governments of developing countries? A strong will for more serious aggressively organized recycling initiatives, embedded with strict regulations supported through...

12 January 2021 510 13 View