I am developing a project with bacteria isolated from the marine environment, in the reactivation process in TSB agar medium they produced a lot of biofilm, and when developing the growth curve in liquid medium with TSB broth they also produced biofilms suspended on the surface of the culture, these are interfering In the absorbance readings for the standardization of the culture and subsequent evaluation of the growth curve, no matter how much I shake it, lumps remain in the culture medium.
Hi Jose,
Have you tried to use different growth media? like Difco Marine Broth 2216 (MB) for example.
I also found very good results using a dilution of Marine broth with seawater. I wanted to simulate the oligotrophic conditions from the seawater, avoiding nutrients limitations and being more comparable with the normal situation the marine bacteria have in the seawater, since the culture media we buy are very rich in nutrients. Maybe, using that diluted media helps you to not having such a biofilm. Check the papers just in case. I hope it helps.
1) CO2leaking from sub-seabed storage scenario: responses of two marine bacteria strains. Ana R. Borrero-Santiago, M. Carbú, T. Ángel DelValls, M. Inmaculada Riba Marine Environmental Research 2016 doi:10.1010/j.marenvres.2016.05.018 (2016)
2) Carbon capture and Storage (CCS) Strategy: a risk assessment focused on marine bacteria. Ana R. Borrero-Santiago, T. Ángel DelValls, M. Inmaculada Riba Ecotoxicology and Environmental Safety 2016 doi:10.1016/j.ecoenv.2016.04.020 (2016)
3) Implications in studies of environmental risk assessments: does culture medium influence the results of toxicity tests of marine bacteria?. A. Díaz-García, Ana R. Borrero-Santiago, M. Inmaculada Riba. Chemosphere. 2018 doi: 10.1016/j.chemosphere.2018.04.066 (2018)
Dear Ana R. Borrero Santiago thanks very much
I will review the findings that you share with me.
Regarding the medium, I have only used TSB, I will explore other mediums as you suggest
Best regards
Estimado Tomas Rivas-Garcia
Muchas gracias por la sugerencia y atención al cuestionamiento. Me genera una nueva duda, el Tween 80 generaría la lisis celular por la disminución de la tención superifical de la membrana celular de las bacaterias? No deseo que las células mueran.
Quedo atento y pendiente..
Gracias
Biofilm formation by marine bacteria can be challenging to disrupt and solubilize in culture media. Here are a few strategies that can be used to solubilize the biofilm:
It's important to note that the effectiveness of these methods will depend on the type of bacteria and the specific biofilm structure. Therefore, it may be necessary to test several methods or to use a combination of methods to successfully solubilize the biofilm.
Harry Kenn Tolentino Dela Rosa Thank you very much for the variety of alternatives you offer, I will consider your suggestions. Kind regards
José Leonardo Ledea Rodríguez I'm glad that you found my answer helpful. Good luck to your project.
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