How can the adhesion between the Teflon coating and the base material be enhanced?

More Jie Tan's questions See All

How to display grain boundaries in RVE model?

Many literatures have shown that the RVE model shows the grain boundaries between different grains. How can this be achieved using DAMASK+paraview?

21 July 2024 9,224 1 View

How to understand Instantaneous SINR (ISINR) ?

I read two papers which uses Instantaneous SINR (ISINR) as criterion for performance evaluation. In the two papers below, it seems the desired signal (numerator) in ISINR does not involve...

16 July 2024 4,545 0 View

Anybody can assist me in Protein and magnetic beads seperation?

Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...

02 July 2024 5,502 4 View

Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase. Which is better?

Been doing searches to find the best paper to start understanding the advantages and disadvantages of each technology: Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with...

29 June 2024 9,856 1 View

Is it better to use 2 intact classes for the quasi-experimental study?

Hi , in my study I will compare the effectiveness of Al generated feedback and traditional feedback in writing lesson.It will be a quasi-experimental design,but l have 3 classes not two.What...

25 June 2024 6,244 3 View

How to distinguish the origin of sulfides in subduction zone interface rocks?

If we find sulfides in subduction zone interface rocks, how can we distinguish whether these sulfides are related to crust-derived fluids or originate from the original mantle wedge? Especially...

15 June 2024 654 1 View

Help with mounting meningeal tissues with Prolong Diamond?

Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...

05 June 2024 624 2 View

How to combine different Effect sizes (OR, RR, HR, PR) & which one to use for Meta-analysis?

Hi all!...

29 May 2024 7,789 4 View

Need assistance in optimizing my protocol. Anyone can assist me about inducing hPSC or iPSC to MSC?

Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....

19 May 2024 3,164 2 View

Request for Assistance with VSH 15-Item Tool Approval and Survey Instrument?

would like to use the VSH 15-item tool to assess sleep quality, but I'm unsure where to obtain approval for its use. I understand that the developer of the tool has passed away. Could you please...

03 May 2024 609 0 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Why is it not common to see similar pairs in polymer sliding pairs?

If the pairs are similar, will it reduce the adhesion due to electric charges?

29 July 2024 3,185 2 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

How do I avoid HEK293 cell lifting during biotinylation?

I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...

23 July 2024 4,740 3 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

Issues in coating of cathode material on Al foil, why we are not getting proper coating and results when the cathode was coated on Al-foil..?

We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...

09 July 2024 5,123 4 View