There is no need to qualify a new method in water as long as you use the current methodology. It is the molybdenum blue colorimetric method using a liquid-liquid extraction (MDL is ug/L). Follow the USEPA or APHA (American Public Health Association) method in its 'Standard Methods for Water and Wastewater'.
http://bfy.tw/CxRW
The colorometric methd is well-established as above. You may also consider an ion selective electrode. ICP.... etc etc
Hi Arun. As others have mentioned above, the colorimetric method is one of the best approaches. If you would like to measure soluble reactive phosphate or total orthophosphate using standard bench-top procedures without a lot of fancy equipment, I have attached a protocol I have used with much success. If you would like to measure Total Phosphorus at some point, I have attached a similar protocol with an extra oxidation step that will convert all of your phosphorus to phosphate first. If you have a flow injection analyzer at your disposal, I have attached a protocol for that as well. Please let me know if you have any questions! Best, Bianca
Dear
The amount of phosphate is determined by molybdenum blue phosphorus method in conjugation with UV-visible spectrophotometer. This method is based on the formation of phosphomolybdate complex with the added molybdate followed by the reduction of the complex with hydrazine hydrate in aqueous sulphuric acid medium at 840 nm in the concentration range 0.1-11 ppm. The optimized concentrations of various reagents used are 0.20N sulphuric acid, 0.02M hydrazine hydrate and 0.20% ammonium molybdate.
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