Hi Biswajit,

Have a look at the following link below which you may find useful -

https://www.nottingham.ac.uk/research/groups/nbic/index.aspx

I am sure there are many things that you can do but you need to be a bit more specific as to what the the target is for instance. If you know what the molecule (i.e protein) on the bacteria that is involved in binding to surfaces for instance and is disrupted when a phytochemical is added - then you can isolate the protein of interest and perform binding studies using ITC, SPR etc with the relevant phytochemical to determine the binding affinity (kD). If the binding data from the ITC or SPR or any other binding method (i.e FRET) is of suitable affinity (nanoMolar for instance) then you can do structural biology (i.e X-ray crystallography) work on it to obtain the 3D structure of the complex - this can allow you to improve on the phytochemical to obtain a much tighter binding by performing structure activity relationship (SAR) .

There are so many other things to do!

hope this helps.

Thank you very much Bubacarr G Kaira . In my case, I do not know where the phytochemical binds. After application of that phytochemical, It shows loss of extracellular polymeric substances and a change in cell membrane integrity.

Now I want to study it to molecular level . what should I do now?

Form the complex of your phytochemical with the target and perform deuterium mass spectrometry to identify where it binds.

You can try looking at the effects of your compounds on the expression of genes involved in biofilm formation.

yes John Paul Matthew Guzman sir, But what are those test should I perform to check effects of the compounds on biofilm formation related genes