As iron oxide nanoparticles are incorporated into an increasing number of medical products subject to FDA regulation, questions about formulation, pyrogenicity, sterility, and sterilization procedures are emerging.
Filtration is the best method so far. Select filter pore sizes that can retain bacteria and some viruses. secondly make sure there are no toxins as well. However make sure you know the surface charges of the filters and the metal oxide nanoparticles.
Thanks a lot; could you please provide us with some information about these filters.
Dear Yahia, what are the sizes and surface charges of your NPs? There are various pore size filters ranging from 3.5nm to .22mm. Chose the one that you know can help in retaining microbes? After that do some quality control (QC) to make sure the NPs are sterile. If you are determining immune profiles and more of IL-12 are being express, suspect toxin presence.
DR CS Yah
Usually they are 8initially sterile due to harsh synthesis conditions. But they can be sterilized by addition of H2O@ or enzymes, which are producing H2O2 e.g. Glucose oxidase:
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Have a look at Melissa et al 2014: Challenges facing sterilization and depyrogenation of nanoparticles: Effects on structural stability and biomedical applications.
Filtration will be the best method, however the surface charges of the oxides nanoparticles and the filter should be taken into consideration. If you need further information do not hesitate to contact me.
Thank you very much for this article and your excellent suggestion, I'll read it and get back to you as needed. .Best regards,
A simple way would be sterilization with ETO according to ISO 11135.
Hope This paper can answer your question, as without sterilization cells can not be grown on newswires:
Article Efficient intracellular delivery of biomacromolecules employ...
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