Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

What do you mean by 'excitation' of a plasma wave? What is the physical significance of this 'excitation'? Why is it necessary?

I have read numerous research papers on various mechanisms of excitation of surface plasma wave. But why do we need to excite a wave? Is it necessary? Can't the wave propagate without being excited?

25 January 2021 3,790 3 View

What is the difference between research summaries and abstracts? Can an abstract be contained in a research summary?

Some articles confuse the two subjects. I need to find out if they can be used interchangeably. If not, what is the difference between the two?

13 January 2021 847 3 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

Do anyone know a lab in Mexico where dielectric properties of solids and liquid can be measured?

I mean those kits that can be connected to vector network analyzers in order to find out permittivity and permeabilitty of solid and liquid from around 1 to 10 or 30 GHz

25 November 2020 852 1 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

How relevant is the Area under curve (AUC) in ROC being different from .50?

I analyze the psychometric qualities of a screening instrument to predict depression after stroke using ROC analysis. In this context, I read several papers which report an AUC without a p-value...

12 October 2020 6,557 4 View

How do you guys measure the rate of protein translation for a specific protein?

I am trying to think about a way to measure the protein translation rate for a single and specific protein that I am interested in. I've been thinking that Ribo-seq might be a good idea for this...

07 September 2020 9,054 3 View

Determination of Lipid A in Salmonella?

Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....

26 August 2020 9,455 1 View

May I know how to determine the acceptable intakes for new types of nitrosamine discovered in drug?

Currently only common nitrosaminse like NDMA and NDEA have mentioned the acceptable daily intake. Based on EMA guidance, should refer to ICH M7 chapter 7.5 guideline. I have read but could not...

20 January 2021 522 1 View

What is the reason of big variations in Biuret total protein assay?

Hello. I used from Thermo Scientific BCA kit and Sigma protein STD 2 mg/ml (0-2000 ug/ml) and prepared a lot of dilutions from my sample (up to 1-64). I had a different final concentration on my...

18 March 2020 488 3 View

What is a self intensity test in STD NMR?

Some papers report performing a "STD self intensity" test to check the aggregation of compounds in STD NMR. Can anyone please elaborate how such a test is performed?

27 August 2019 3,202 2 View

What is a self intensity test in STD NMR?

Some papers report performing a "STD self intensity" test to check the aggregation of compounds in STD NMR. Can anyone please elaborate how such a test is performed?

07 August 2019 6,832 2 View

Trouble With Data Analysis?

How does one get around performing a 2 Proportion T-Test in SPSS (A dichotomous variable is being measured between two groups)? Doing a Chi-Squared will not provide the mean difference with either...

22 July 2019 3,896 3 View

Trouble With Data Analysis?

How does one get around performing a 2 Proportion T-Test in SPSS (A dichotomous variable is being measured between two groups)? Doing a Chi-Squared will not provide the mean difference with either...

06 July 2019 6,160 3 View

How to interpret coefficient in regression when the independent variable is a change in %?

Good morning all, I am having some trouble interpreting my regression output. My model is LN of transaction volume = alpha + beta1_RER + control variables RER is defined as the change in the...

28 April 2019 516 3 View

How to interpret coefficient in regression when the independent variable is a change in %?

Good morning all, I am having some trouble interpreting my regression output. My model is LN of transaction volume = alpha + beta1_RER + control variables RER is defined as the change in the...

03 April 2019 2,215 3 View

ICH QD about matrix design ?

how to get matrixing design's test lists ?

31 December 2018 4,346 0 View

Is a serial dilution approach appropriate for developing/validating HPLC impurity method linearity/accuracy/range?

11 December 2018 3,755 14 View