Which is best tool for alignment of large bacterial sequence?

Hi! I need to align big fasta files (bacterial 16S) . I have tried to align them in some web-servers and freeware, but my files are too big to be run in there. I will be very glad if you could...

16 January 2021 1,266 4 View

Can I fix my samples in FAA fixative solution for about 6 months?

Dear researchers I have some samples from the stems of watermelon plants and I want to fix them in FAA fixative solution for about 6 months; then I will dehydrate, embed, and stain them for...

06 July 2019 8,104 10 View

How can I calculate light spectrum without using spectrometer?

Dear researchers, I'm working on the effects of different LED light qualities on growth and development of grafted watermelon seedlings during healing and acclimatization period. The problem is...

05 June 2019 9,261 8 View

Why the brain of an adult can't regrow the same cells when damaged?

I know that an infant's brain can repair itself when damaged but why doesn't the same happen in adults after stroke or brain injuries?

17 October 2018 9,337 4 View

How i do use DNS method to fungal Pectinase activity assay?

Thanks.

10 November 2017 755 0 View

How to determine the degree of similarity between two signals?

I'v been doing an investigation about detection of a specific signal and I need to determine that how a received signal resembles the reference signal. A primary approach to this problem is done...

06 November 2017 6,369 7 View

Can anypne share with me the GAMS code for the backward-forward load flow for distribution systems?

I need the GAMS code for distribution load flow. In this regard, I have found the (improved) backward-forward sweep suitable to my work. So I need the GAMS code for this method. I would appreciate...

14 June 2017 5,999 12 View

How can I fit concentration profiles of carbon (introduced by Wynn et al, 2004) to my data?

06 April 2017 2,991 3 View

How can we determine representative volume element length (Lrve) for concrete?

for periodic materials, determining Lrve is simple but for a non-homogeneous material such as concrete, it is not that simple. any advice such as methods, references, etc. would be appreciated.

04 November 2016 6,044 3 View

Who can send download link for below software MBF_ImageJ_setup.exe?

I cant found download link.

31 December 2015 3,877 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View