Dear Shahrooz,

Please read the following text:

CEP4 Plasmid | Cell-culture Database - TOKU-E

cell-lines.toku-e.com › Search by Plasmid/Vector

Cells name, Plasmid/Vector, Antibiotic, Concentration, References. A9 · pCEP4 ... Hygromycin B. 260μg/ml. 964 · Jurkat, Clone E6-1 · pCEP4. Geneticin (G418).

USE THE FOLLOWING LINK FOR CONCENTRATIONS:

http://cell-lines.toku-e.com/Plasmid_1582.html

Cells name Plasmid/Vector Antibiotic Concentration References

93 pCEP4

Hygromycin B

260μg/ml 964

E6-1 pCEP4pRcCMV-dnLMW-PTP

Geneticin (G418)

500μg/ml 176

Hoping this will be helpful,

Rafik

Dear Rafik.

this vector has a main body as I could understand pRc/CMV; then, were sub-cloned into pcep4. Therefore, it has two selection markers; G418 from the first vector and Hygromycin from second one.

Dear Shahrooz,

The Gentticin is a replacement of Hygromycin B. Please see below the details of each one .

Jurkat, Clone E6-1

Species source: human

Organ source: T lymphocyte; acute T cell leukemia

Plasmid:

pCEP4

pRcCMV-dnLMW-PTP

Culture/Media: RPMI 1640 Medium

Antibiotics:

Geneticin (G418) - 500μg/ml -

Reference:

L. H. Butterfield, A. Koh, W. Meng, C. M. Vollmer, A. Ribas, V. Dissette, E. Lee, J. A. Glaspy, W. H. McBride and J. S. Economou 1999. Generation of human T-cell responses to an HLA-A2. 1-restricted peptide epitope derived from -fetoprotein

Is a replacement of:

293

Species source: human

Organ source: kidney; fetal

Plasmid:

pCEP4

Culture/Media: Dulbecco's modified Eagle's medium

Antibiotics:

Hygromycin B - 260μg/ml -

Reference:

J. Ma, J. E. Tasch, T. Tao, J. Zhao, J. Xie, M. L. Drumm and P. B. Davis 1996. Phosphorylation-dependent block of cystic fibrosis transmembrane conductance regulator chloride channel by exogenous R domain protein.

Hoping this clarifies the point,

Rafik