How can I split a Gromacs .tpr file?

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Is there any free Journal to publish systematic literature review protocol? I have checked JMIR but they are also charging for protocols?

I am looking for a free Journal (good impact factor) to publish a systematic literature review protocol. I have checked a few but they are charging a lot. Any recommendation will be appreciated....

19 November 2023 9,285 2 View

How we do sequence analysis for insert?

What does it mean sequence in frame. How we do full read of sequence.

16 October 2023 4,139 1 View

Modelling V2I scenario in MATLAB?

Can any one please share the MATLAB code for V2I scenario?

10 August 2023 8,439 0 View

If I have scale dependent variable and ordinal independent variable, what can be the possible options of tests for research in SPSS?

Can I do regression analysis for such kind of variables?

02 August 2023 3,619 3 View

Why F1 particles are only present in inner membrane of mitochondria not in outer membrane ?

what are the functions of F1 particles in mitochondria ?

24 July 2023 230 3 View

Is there literature suggesting to exclude item with negative point-biserial in CTT first before we perform item analysis with IRT approach?

I have been suggested to exclude item whose point-biserial correlation coefficient (indicates that this item fails to discriminate student competence, based on CTT) is negative before we perform...

12 June 2023 886 0 View

How can I Synthesize 1-bromo-2-hexanol in good yield? and how to purify the reaction mixture?

I am following this protocol (attached) but I get more of side product (bromo hexane) and very less amount of 1-bromo-2-hexanol.

07 June 2023 6,169 0 View

I wanted to know if there is any non-invasive way to differentiate between male and female Aedes larvae?

Is there a non-invasive method available to distinguish between male and female Aedes mosquito larvae? I would greatly appreciate any information on this topic.

14 May 2023 7,386 4 View

How to use ANN for accurate text classification?

I have a dataset with textual data and four class labels. I want to use ANN for performing accurate text classification. I have tried to use a neural network for the dataset but it is giving me...

08 March 2023 9,572 4 View

Training opportunities in Neurophysiology and Systems Neurosciences?

I am looking for training opportunities in Neurophysiology and Systems Neurosciences Please connect with me to discuss. Thanks!

08 November 2022 1,612 2 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out?

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...

31 July 2024 781 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View