How can I solve my problem with colocalization using anti CD3 and CD8 antibdoy in mouse intestine cryosections?

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Oscar L. Sierra

It would be a good idea to look at the pictures since CD3 is a general T-cell marker so certainly many more cells will be positive with this marker than with CD8+ which will stain is a smaller subset. Are you doing immunofluorescence or peroxidase-amplification with stains visible in brightfield? -

Prem Prakash Tripathi

Only a fraction of CD3+ cells will be colocalize with CD8+ but not all. Try confocal with maximum projection in case you care unable to find that with wide field.

Gaetana Costanza

Try to use

Goodwin G. Jinesh

It might be Tregs. Try CD24 in addition.