How can I simulate or create a test bed for an authentication protocol in IoT?

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What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Which phosphor antibodies to choose to study the hypo and hyperphosphorylation state of the SRSF1 protein?

I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...

11 February 2024 7,242 3 View

Does heating of plants extracted solutions in different solvents such as NaCl or NaOH induces protein-polysaccharide formation?

Greetings everyone. I have a question regarding protein-polysaccharide complexes from plants. Recently, I heated plants extractions in NaOH and NaCl. During heating process, the colour turns from...

09 January 2024 3,197 2 View

Is it necessary to change media after transfecting cells with Lipofectamine3000?

I transfected HCt116 cells with GFP using Lipo3000. I didn't change media after transfection as the manufacturer guidelines say not necessary. However, I noticed a change in the cell morphology...

28 April 2023 6,071 6 View

Is Native RNA immunoprecipitation performed without cross linking?

I am trying to study the RNA-protein interactome of a specific protein using IP studies. I need to use native IP for my purpose and wanted to know if native RIP is without cross-linking study and...

01 February 2023 915 0 View

Is loading equal volume of protein in SDS PAGE correct?

I am comparing the protein expression levels in subcellular fractionations (Cytoplasm and nuclear). If I load an equal volume of both extracts (15 ul each to individual wells), is this still valid...

26 January 2023 6,257 3 View

Is the intra-cluster nodes are affected the transmission speed of the communication?

In general, the communication between the inter-cluster nodes has some linking issues due to the behavioral attributes of the sensor nodes. Hence the delay occurred to transmit the message or...

12 September 2022 2,263 0 View

What is the right media for HCT116?

Is DMEM or DMEM/F-12K the right media for culturing HCT116 cells apart from McCoys media? Did anyone use the gibco one? I see a lot of dead cells when using DMEM. It would be helpful if someone...

20 June 2022 1,059 3 View

How is FPKM calculated from raw gene count file?

I am working with RNAseq and qRT-PCR validation results to see a correlation. I am comparing a diseased group to a healthy group of cells. I have 4 biological replicates in each category. However,...

03 June 2022 7,982 5 View

How to extract the time series for each voxel in CONN?

I'm trying to find the output file which contains the time series for each voxel, after denoising. In the 1st level analysis in the GUI, I have the option of clicking on a given voxel for a given...

21 December 2021 429 1 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

When I simulate for S parameters using ADS, I have faced this issue that I have attached. Please anyone clarify me?

...

31 July 2024 1,671 3 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View

What are needed modules for an IoT waterlevel monitoring system?

I want to know the modules needed for an IoT project for water level monitoring

27 July 2024 1,502 3 View

Why microbes are used in environmental engineering and role of microbes in reducing environmental pollution and improving environmental quality?

25 July 2024 5,192 5 View