I intend to separate ribosomal and non-ribosomal fractions of cytosol using 20% sucrose cushion. I have already run the cell lysate on top of the 20% sucrose solution for 2 hrs at 1,39,000 g. I could not find any separate layer of ribosomal fraction or did not get any pellet. Should I use fractionator to separate the fractions? Please provide me a suitable procedure.
Thanks in advance.
Dear Anindhya Sundar Das
Pls. find the attached files, may help in your research, good luck.
http://nar.oxfordjournals.org/content/early/2014/01/21/nar.gkt1404.full
http://jcs.biologists.org/content/joces/112/5/681.full.pdf
http://www.jbc.org/content/275/43/33820.full
http://nar.oxfordjournals.org/content/early/2014/12/18/nar.gku1310.full
http://jcb.rupress.org/content/67/1/1.full.pdf
http://faculty.washington.edu/dmorris/Protocols/Mammalian_polysomes/Ruan%20et%20al.pdf
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189431/
http://derisilab.ucsf.edu/data/pdfs/MembraneBoundPolysomes.pdf
Robert A. Meyers 1995. Molecular Biology and Biotechnology: A Comprehensive Desk Reference John Wiley & Sons,1084Pp. ISBN0471186341, 9780471186342
Regards
Prof. Houda Kawas
Respected Prof. H Kawas,
Thank you very much for your kind help.
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