9 Questions 13 Answers 0 Followers
Questions related from Anindhya Sundar Das
Dear all, We have introduced double stranded break in our gene (exonic) using a single gRNA. We have also checked efficiency of the cut by T7E1 assay. Now we are trying to genotype invidual clones...
06 June 2018 5,406 3 View
Hi I am interested to check a particular mRNA and a protein interaction/ localization inside cells. For this reason, I want to tag the transcript. Beside RNA-FISH or any such hybridization...
11 November 2017 8,742 0 View
Hi..is there any literature on successful gene knock out in THP-1 monocytes via CRISPR-Cas9 using Lipofectamine RNAiMAX? Thanks in Advance
03 March 2017 4,751 1 View
Hi..I have over-expressed a protein (tagged with His) via retroviral transduction method in THP-1 cells. Now I am interested to check if there is any binding partner with my tagged protein in a...
12 December 2016 2,712 4 View
I intend to separate ribosomal and non-ribosomal fractions of cytosol using 20% sucrose cushion. I have already run the cell lysate on top of the 20% sucrose solution for 2 hrs at 1,39,000 g. I...
06 June 2016 1,163 2 View
I am interested in searching the gene expression profile of a particular set of genes in a specific disease condition like atherosclerosis from NCBI GEO database. As I have almost no background in...
07 July 2015 662 7 View
I have converted THP1 monocytes to macrophage using 5 ng/ml PMA for 48 hrs in complete RPMI1640.PMA Containing media was discarded and cells were washed with sterile DPBS for 3 times and given...
04 April 2015 5,149 2 View
I am culturing THP1 cells in RPMI 1640 (as advised by ATCC formulation, except 2ME). Unfortunately my cells are forming clumps a few hours after seeding (at 1* 10^5 cells/ml) before even going to...
03 March 2015 214 10 View
I have differentiated THP1 monocytes into macrophage using 5 ng/ml PMA for 48 hrs. Then after washing twice with DPBS i have kept the diffrerentiated cells for 3 hrs in serum free media. Then I...
03 March 2015 1,359 8 View
