Greetings,
I am here to take an expert help from all the respected researchers and scientists here. I am working on antimicrobial peptide hydrogels. I have designed antimicrobial peptides library and predicted their antibiofilm activity through self-build ML prediction model. My aim was the formation of peptide gel. But I am failed to do so.
For your expert opinion, I would like to mention few important properties of my peptide. Well, it is designed in such a way that it has higher hydrophilicity, it's basic and positively charged. It contains 12 amino acid residues, and it is KLR rich peptide. Theoretical/predicted Isoelectric point is 12.45. So far, I have tried high as well as low pH stimulus. I have also tried freeze thaw cycles. But it is not getting gelatin structure. It is important to mention that I am trying on concentration of 5mg/500ul (also as 10mg/ml).
Now, I am going to try to give ionic pressure by the addition of salts such as MgCl2, CaCl3 etc.
I would like you scientific community to help me in this regard. Ask me questions so that we could have more clarity.
Hello, I would suggest to go higher with concentration o the peptide to proove the concept. may be freeze thaw cycle in presence o gluconolacton, it did woked well in our case o self-assembly. Article Formation of macroporous self-assembled hydrogels through cr...
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