How to extract peaklists from mass spectrometry raw file and do mascot search for my protein in ncbi?

I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.

30 January 2021 3,081 3 View

Which heating methods do you recommend for establishing a poultry house (oil heater, gas heater, electric heater)?

I think a gas heater is better for the lower operating costs

03 January 2021 385 18 View

What is microbiology and its types,scope?

the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...

13 December 2020 8,287 6 View

How can one calculate Gibbs energy and entropy in ab initio molecules dynamics?

Hello, I've been investigating a bunch of monomers which spontaneously react in water to form a polymer. I would like to know how to calculate the thermodynamics of such a system. On the surface,...

18 November 2020 5,595 3 View

How to make a solution of resazurin powder ???

I want to test chitosan nanoparticles as antimicrobial against pseudomonas How to use resazurin microtitre assay????

08 November 2020 9,533 2 View

Please suggest me EI compendex conferences with good acceptance rate?

I want to publish a paper at the EI-Compendex conference, please suggest a conference with a high acceptance rate. My field of research is IoT security and cryptography analysis.

19 October 2020 5,365 3 View

Why COS 7 Cells change its morphology to round shape (rounded nucleus and peri nucleus cytoplasm) after Transfection?

I have transfected COS 7cells to express Protein-GFP conjugate, transfection rate was very low (20-30 %) , but the cell morphology changed, many of the cells appear round, (nucleus round too)...

15 September 2020 5,324 3 View

How to separate mixture of two organic compounds using size exclusion chromatography?

My organic compounds have a molecular weight in the range of 2500g/mol and other is 1066.22 g/mol. aThe TLC results show three spots. But the main problem with my compounds are acid sensitivity of...

20 August 2020 6,119 3 View

Dear All. Can normal RNA extraction kit be used for RNA extraction from cell-free medium like culture supernatant, VTM, plasma, etc.?

I am asking specifically for RNA extraction for VTM containing COVID-19 SARS-CoV-2 virus particles. Will the extraction kit make difference in total or specific (viral) RNA yield? There are many...

16 August 2020 8,385 2 View

Should outlier analysis be done before PCA while trying to perform step-wise Regression? Also how to deal with outliers?

Currently I am working on a project wherein I need to predict the estimated new Selling price of IPL players based on their current base price and previous years' performance & sold price. I'm...

05 August 2020 4,254 6 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View