When DMPO enters cells it is reduced so its ability to spin trap is reduced. In order to thwart this effect, I am searching for a molecule that might enter the cell and oxidize the reduced DMPO.
I am not sure this is either possible or advisable. Anything that affects DMPO will also very likely affect cytosolic redox state, and the radicals you are trying to measure. Any results would therefore probably be uninterpretable.
Hi, The reduction potential of DMPO is too low to be be reduced by cells, but the DMPO radical adducts are rapidly reduced. Ferric cyanide has been used to reoxidize the hydroxylamine back to the spin adduct in bile and a few other cases, but not in cells.
Hm... This is unusual... Let's try with the simplest solution: how concentrated those cells are? How much mW have you applied? Accumulate the signal for 15+ min. What percentage of trap was used (be generous, always add more)... Are those cells still alive *(my cells were almost dead)? Is there something strange in the medium?
More DMPO related Q&A - on my blog:
https://steemit.com/steemstem/@alexs1320/answering-9-rg-quest
Dear all, thank you for your answers.
As I am not working on this project anymore I do not need help but feel free to continue the discussion on this topic.
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