Need to measure proteomics of the chloroplast but with Rubisco is such excess I won't see the lower abundance proteins, therefore need a method that can remove Rubisco in a consistent way.
Gustavo Acevedo-Hernandez
Here are two options: antibody-affinity column to trap Rubisco: http://nhjy.hzau.edu.cn/kech/yyzw/other/images/proteomic/Proteomic%20analysis%20of%20rice%20seedlings%20during%20cold%20stress.pdf
Alternatively, aggregation in the presence of high concentration of DTT:
http://link.springer.com/content/pdf/10.1007%2FBF03036130.pdf
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Rameswar Prasad Sah
http://14.139.232.166/opstat/index.asp
You can analyse your data
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