Hello Barath!

Although I'm not as experienced, have you tried prolonged centrifugation in CsCl? This is referred to as Gradient or Differential Centrifugation. This is one method of isolating chloroplasts. I know that is not exactly what you are trying to do, but I think if you can remove the chloroplast intact, you may be able to remove the chlorophyll, too. See THIS link below or check out the article I've attached. 

Also, are you modelling your extraction after another proven protocol? What compound are you trying to extract? Are you doing HPLC? See below, and hopefully this will point you in the right direction :)

http://www.ncbi.nlm.nih.gov/pubmed/24408195

hi Kauahi

 thanks for your reply,  it really meant  a lot to me , 

here actually  chlorophyll is my undesired product   it is really  making my assay difficult . so i need to remove it so that my assay would become  easier and also successful . 

You should tell us why do you need to remove the chlorophyll. For instance, if it interferes in spectrophotometry, you may try to bleach it using strong light or to displace Mg with an acid (also EDTA; light will accelerate it).

saponify the extract adding it with KOH  and holding the solution on a water bath for 2h. then add the obtained solution with any organic solvent, which is insoluble in water. After this saponified chlorophylls is easy to remove by rinsing  with water.

Try dioxane precipitation of Chls if your compound of interest is not affected.

Can you be more explicit in the object of the research.

add charcoal powder to the extract and centrifuge the mixture 

Check these threads also

https://www.researchgate.net/post/How_could_we_remove_chlorophyll_from_plant_extracts_during_purification_of_natural_compounds?tpr_view=cf963c49-6fdb-43d5-a01f-f1e47d31e8b6_1

https://www.researchgate.net/post/How_can_I_remove_chlorophyll_from_leaf_extract

https://www.researchgate.net/post/I_would_like_to_know_about_the_method_of_separating_chlorophyll_from_ethanolic_extracts_from_celtis_africana#557153915dbbbd835b8b4571

https://www.researchgate.net/post/How_can_I_measure_the_concentration_of_the_chlorophyll_in_the_eluent?tpr_view=8d0fb62a-4df7-4d4b-a492-edd46f43f3cb_2

https://www.researchgate.net/post/Can_I_use_oven-dried_plants_for_chlorophyll_Soxhlet_extraction?_trid=55409d7ad2fd64cb628b45b0_

how DMSO helps in chlorophyll extraction.may i know the principle of it?

What is it that you are actually interested in? is it a low molecular compound or a protein? I need to know that before suggesting anything. some of he procedures mentioned above may succeed in removing chlorophyll but may remove or damage the molecule of your interest as well.

In my view, the best ways is by isolation of compound to be studied using specific procedures.  For example, specific procedure to extract sugar from plant material.    reference; Foley et al. 1992. Carbohydrate status in dormant and afterripened excised wild oat embryo. Physiol. Plant. 85:461-466.

Chlorophyll is a green pigment that is present in all photosynthetic plants, as well as in some bacteria. It occurs in three forms, all of which are magnesium-centered porphyrins containing a hydrophilic carbocyclic ring with a lipophilic phytyltail. Chlorophyll is a photoreceptor up to a wavelength of 700nm; it is sparingly soluble in alcohols; and its solutions are blue-green in color. Chlorophyll often interferes with the analysis of bioactives in natural product research or in pesticide analysis. Discovery DPA-6S polyamide SPE products can be used to remove chlorophyll from aqueous or methanolic extracts in these applications. In this experiment, 300mg of bulk DPA-6S sorbent was packed into 3mL SPE tubes and plant extracts were processed through the tubes.

If you do not extracted hydrophobic compound, you can try isoamylalcohol

Pass your diluted extract through activated charcoal, repeatedly, 2 or 3 times, if need be!

In my expert, the best method for removing chlorophyll is SPE (10 g) for 1g extract

Please check this article. The safe method was developed and optimized.Article A green and effective method using oils to remove chlorophyl...

Found this thread when looking up methods to remove chloroplasts. The fluorescence contaminates spectra analysis for heme concentration. For SPE and charcoal powder, could these methods also interfere with heme concentration?

Sarah, your question isn't clear. Were you looking for a method to remove chloroplasts or chlorophyll? SPE and charcoal are not likely to interfere with heme concentration. However, since I am not sure about this, you can refere to the following:

https://www.sciencedirect.com/science/article/pii/S1464345603800145

Article Extraction and purification of high-value metabolites from m...

Article Rapid and quantitative extraction method for the determinati...

Extraction with NADES can degrade chlorophyll without loss of target compounds. Article Development of a colorless Centella asiatica (L.) Urb. extra...

Extract with n-hexane followed by water. It will remove chlorophyll without removing secondary metabolites, especially which are methanolic in nature. 

by using n,n-dimethyll formamid soultion