My sample extraction is done by Ethyl acetate , now i am facing problem while doing TLC profiling so how can i remove chlorophyll.
Two possible approaches. One is remove by adsorption to a matrix like Kieselguhr or use a solvent system in TLC that will retain the chlorophyl at the origin while allowing your other components to migrate. You will also have to use different types of plates. The other option is to use an HPLC system to optimize the conditions for removing this component.
I think that the bacteria might metabolize the other organic compounds present in the plant extract resulting in the production of organic acids in the medium. as by products
what about using charcoal for adsorbing that chlorophyll.
I did by charcoal already and its showing quite good result but its not remove completely , and biological method wont be a good idea i think so , but still let me try it once.
i put activated charcoal with Ethyl acetate solvent in my extract and kept it for 2 days and now i got a complete clear solution. i think not only chlorophyll my other components also absorbed by charcoal.
can anybody suggest that in what ratio and the time duration of charcoal treatment for chlorophyll removal process.
Mr Swain you may remove all the adsorbed components using polar organic solvent like methanol or acetone from the charcoal by simple treatment. Then again treat the extract with charcoal for shorter duration making chlorophyll to be adsorbed.
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology