Hi,
i have done a GC-MS and LC-MS analysis of a plant extract , while interpret of LC-MS data i am finding difficulties, so could anyone please suggest me a way or provide me some information which could help me for the data interpretation.
thanks.
What difficulties are you having?
Things I suggest are a literature search of the plant and related species to see what has already been found, and tentatively identify peaks. If you chose a portion of the extract to run on GC/LC-MS due to biological activity, also do a literature search on that activity to see what sort of compounds exhibit activity. for example, my experience is that central nervous system assays often were positive for alkaloids, although there are exceptions (cannabinoids, for example).
Remember that adducts may have formed (sodium, potassium, solvent), so you have to cognizant of these as well. Also remember some groups may be easily lost (OH as water, carboxylic acids as CO2, and so forth). How are you ionizing the compounds? ESI? APCI? EC? Steroids are often not found with ESI, but show with APCI with loss of water.
What difficulties are you having?
Things I suggest are a literature search of the plant and related species to see what has already been found, and tentatively identify peaks. If you chose a portion of the extract to run on GC/LC-MS due to biological activity, also do a literature search on that activity to see what sort of compounds exhibit activity. for example, my experience is that central nervous system assays often were positive for alkaloids, although there are exceptions (cannabinoids, for example).
Remember that adducts may have formed (sodium, potassium, solvent), so you have to cognizant of these as well. Also remember some groups may be easily lost (OH as water, carboxylic acids as CO2, and so forth). How are you ionizing the compounds? ESI? APCI? EC? Steroids are often not found with ESI, but show with APCI with loss of water.
Hi, Jack Silver Sir
thanks for your valuable suggestion, i have ionizing the compound by ESI.
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