Can I inactivate restriction enzymes after freezing the digestion product?

I performed a digestion that ended up at midnight, but forgot to inactivate the enzymes (HaeIII and RSAI) before freezing the products. I plan to run a gel to differentiate yeast isolates. Can I...

09 December 2020 3,551 3 View

Frozen spheroid section is not staining (fluorescent + H&E), any ideas?

I have cryosectioned a spheroid which was grown in collagen and agarose and then embedded in OCT Tissue Tek solution before freezing in a dry ice ETOH bath of liquid N2. The sections are very...

08 November 2020 3,299 3 View

Anyone with experience on DNA extraction from biopsies for microbiome analysis?

We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...

24 September 2020 6,845 4 View

Has anyone used a Phalloidin stain that permeates the cell membrane of living cells (rat cardiomyocytes)?

In this particular experiment, it is not possible to perform any permeabilization protocol that weakens the sarcolemma or cytoskeleton, so I would have to rely on finding a Phalloidin that can...

09 January 2018 2,642 4 View

Any tips in conducting the sulfide method using the 'Standard methods for the examination of water and wastewater'?

23 January 2017 3,448 3 View

How do you calculate N2O and CO2 flux emissions collected at different time? What equation(s) would you suggest?

This is a soil incubation experiment in situ. Collection occurred throughout a period of time. Please provide ideas of one or more equations that you could suggest to transform my values into...

21 September 2016 6,857 4 View

Are there any other tracking methods aside from satellite tagging that are just as efficient in tracking the long distance movements of whale sharks?

The migratory patterns of Rhincodon typus is very interesting to me and I have read that satellite tagging is the method typically used when tracking their movements. However, I would like to know...

07 July 2016 3,382 2 View

How to deal with mixed methods studies in mixed methods synthesis?

I have been reading about mixed methods synthesis techniques (Sandelowski et al 2006, 2012 and Joanna Briggs Institute 2014) for systematic review. I think a segregated synthesis approach will...

05 June 2015 138 5 View

What is the minimum fractionation method that is needed for whole mammalian cell lysate prior to Orbitrap-MS?

01 February 2015 9,914 5 View

Does anyone know of a replacement product for Whatman UNIFILTER 96 well plates (cat no 7700-3305)?

28 August 2014 5,463 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View