What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,005 2 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,226 1 View

Transfection in HFL-1 cells?

I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...

17 November 2019 484 2 View

Is there a full list of scales from the FOCUS Questionnaire (van Muijen et al., 1999)?

Hi! I'm currently working on my Master's thesis and looking for the evaluative scales for the innovation and rules value orientation from the FOCUS Questionnaire developed by van Muijen et al....

05 May 2019 4,039 3 View

I have putted the 10fold amount of 2-Mercaptoethanol in my stripping buffer by mistake. Does anyone have experience with such a mistake?

I think my proteins on the PVDF membrane are gone, because there is nothing to detect anymore. But on the other hand the membranes smell after a lot of washing procedures still of 2-ME. Maybe my...

18 July 2018 171 3 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

Can anyone provide advice on how to model age captured in categories within SEM?

10 July 2015 4,972 15 View

Is there any recent work on literary language (or the nature thereof) or of "literariness" more generally?

Critics don't seem to be interested anymore in distinguishing between ordinary and literary language, but I'm wondering whether this is a crucial issue we need to return to.

06 October 2014 8,200 5 View

Can anyone suggest a material with the highest specific heat capacity (higher than water)?

Hi, I was wondering which material has the highest specific heat capacity? Most lists I could find have water at the top with 4.182 kJ/kgK. But I was wondering if there is a solid or liquid...

17 September 2014 5,850 3 View

What is the best way to differentiate osteoclasts out of murine bone marrow cells?

Dear all, We have some problems with generating osteoclasts out of murine bone marrow cells. So I would like to ask in general: - Do you pretreat the cells before you culture them? - Which medium...

04 August 2014 9,354 4 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View