I am currently perfoming multiple brain microinjections in male SD rats over a number days. Unfortunately, I am finding myself needing to replace cannulae obturators (currently using 33G wire) almost daily as the rats are very skilled at removing them. As a result, many cannulae are becoming blocked. Can anyone recommend ways to construct obturators that may reduce the amount being replaced and ultimately, the number of blocked cannulae? Thank you in advance.
PlasticsOne (http://www.plastics1.com) make a nice set of guide cannulae, with stylets and screw-on caps. Blocking is still occasionally a problem, but we found that if you take the cap off and gently move the stylet in the guide cannula every day, the risk of blocking is greatly reduced.
Best wishes
Chris
It is recommended that the wire be bent to around 150 degrees, and then cut, leaving a protruding end of just about 2mm, which is later covered in polyethylene tubing after the acrylic anchors set.
Hi Melanie,
in my practice i used to insert in it a piece of fisching line that i removed at the time of micorinjections.
Alternatively you could use tubing adapter from Microbiotech.
Best whises
PlasticsOne (http://www.plastics1.com) make a nice set of guide cannulae, with stylets and screw-on caps. Blocking is still occasionally a problem, but we found that if you take the cap off and gently move the stylet in the guide cannula every day, the risk of blocking is greatly reduced.
Best wishes
Chris
Indeed PlasticsOne guide cannulae worked well in my experience. Or you can try these:
http://www.microdialysis.se/us/products/probes/gude-cannulae
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