According to literature, Daucus carota can be used for the reduction of some ketones, we tried with or without a buffer (pH = 7), with or without an organic solvent, but it did not work; We mix an amount of the substrate (tens of mg) with a larger quantity of carrot (tens of grams) mixed in the buffer (or water). The reaction is stirred (over 24 hours) and followed by TLC, which always shows the starting material. I wonder how this reaction is reproducible. Could someone correct us our protocol or give us another. Thank you in advance for your answers
Presume you're referring to J Org Chem. 2002 May 31;67(11):3900-3.? The sourcing and preparation of the carrot cells is paramount; did you use (dead) commercial carrot roots? Depending on your seasons, other Daucus species tissues may be available to you (as wildflowers), also (D. muricatus, crinitus, sahariensis, etc.).
Hi Stanton de Riel
Thank you very much for your reply. I am referring to Tetrahedron: Asymmetry 13 (2002) 2299-2302 (and others about whole cell-biocatalysts) . yes, we used carrots purchased in the local market. I had not yet read anything about the wild flowers for the biotransformation of organic compounds. any reference would be very helpful. Thanks a lot.
naima
Please follow the below link to get some more details.
http://link.springer.com/article/10.1007/s10529-005-5187-y
- Badges
- Science topic