Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

Methods for bird trend analysis?

What would be the best method for analysing trends in species abundance in a single area in Latin America over a 15 year period? Could TRIM be used for this purpose? I don't have data about...

27 October 2020 7,532 5 View

How to adapt 280 nm absorbance for protein quantification to microplates ?

Hello. I want to adapt the 280 nm absorbance method for protein quantification of cell lysates to the microplate set up. I learned that for 1 cm quartz cuvettes a conversion factor of 1.3 is used...

06 July 2020 1,818 3 View

Can 6M Urea inhibit lysozyme action? or this happens the other way around?

I mix both components together with other substances to treat my bacterial pellet and extract protein, but I don't know if Urea 6 M or lysozyme loses its effect

24 April 2020 4,098 14 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

How to get forces in a constraint in Abaqus?

Hello everyone! I am doing a static analysis in Abaqus. It's a quite simple model. Two plates connected through to holes using rigid body connections (see attached image). One plate is fixed and...

24 March 2020 3,029 3 View

How to demonstrate (derivation) that the viscous stress tensor is a linear combination between viscosity and velocity gradient ?

Derivation of stress tensor in terms of viscosity and velocity gradient.

08 September 2018 3,587 0 View

How can i reduce computational time for a RK4 programing for a system of 7 ODE with 7 variables (plus time) ?

I make the program for RK4 and, i run it 30 min ago and it is still running. I really need to reduce the computational time.

23 March 2018 1,240 2 View

Goverment equations for Gas compressible flow in a pipe with cooling jacket?

I have to model the Temperature and Pressure profile for this system.

01 February 2018 8,877 0 View

It is possible to detect (by WB) a membrane receptor, mainly in the cytosolic fraction after a differential centrifugation?

07 December 2015 5,342 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View