Hi. I want to remove oxygen inside the water to do some anaerobic incubation experiment. I found some papers purge water with N2 or He to remove dissolved oxygen but no idea how to do that. Is anyone try this before? Thanks!
Dear Jaehyun Lee,
the easiest way to remove oxygen is bubbling nitrogen or helium gas through it. For that you should use a filter cap for the gas tube in the flask to reduce bubble size. See for example this webpage for such kind of setup (it's somewhat more sophisticated, but bubbling alone (for at least 30-60 min before the experiment) will already suffice for most applications):
https://www.shimadzu.com/an/service-support/technical-support/analysis-basics/lib/lctalk/s5/055.html
Some more methods can be found e.g. in this question discussion:
https://www.researchgate.net/post/Liquid-degassing-Whats-better-nitrogen-or-vacuum#view=5cabb315d7141b310264ed63
Best regards
Johann
- Badges
- Science topic
- Similar topics
- Papers