What are the best references for an approach to teaching Differential Equations?

Differential equations are known to be potential tools for modeling and predicting recurrent phenomena in time and space, whether simple or more elaborate models. In this sense, how to approach...

18 December 2020 1,750 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

How many journal articles should come out of a doctoral thesis?

Is it normal if I publish my doctoral thesis into only one journal article (since my doctoral thesis will be data-heavy and theory-light)? Or are doctoral theses expected to be equivalent to 3-4...

01 December 2020 3,803 14 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

Can I compare data from ion exchange membranes with data from ion exchange resin when determining nutrient supply rates in soils?

Hi everyone, I am trying to decide if I can compare nutrient supply rates between two different methods of collection. Method A uses ion exchange membranes and method B uses ion exchange resin in...

23 September 2020 4,535 1 View

Why would a validation error (confusion) matrix return null in Google Earth Engine?

I am working on validating a collection of images from a time series in Google Earth Engine and the confusion matrix that results from validation of the trained image v.s. the landcover classes...

09 September 2020 7,246 3 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

No title

Nutritional science has provided functional foods with the required amounts of natural antioxidants, vitamins, fats, proteins, polyphenols, carbohydrates, and other components required to maintain...

03 June 2020 7,377 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

How to Remove different version of atom from pdb Crystal Structure for Docking Preparation?

I am new to Docking and MD, and currently try to catch on it. I try one of the webserver for docking, but unfortunately not working. it says " Your PDB contains multiple forms of the same residue...

08 February 2021 7,426 3 View

How do you get around this AutoDock Vina error?

I get the following error messages after running the simulation: Sorry, there are no Gasteiger parameters available for atom ligandx.ligand:A:lig1:O7 AutoDock Vina ligand docking initiated for...

26 January 2021 3,822 3 View

The dimensionality of protein and ligand is reduced after docking?

I used autodock to complete the docking between the ligand and the protein. I have a question, why the dimension of the result after docking is much smaller than the dimension of the protein...

24 January 2021 7,363 3 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

Servers for finding free energy and dissociation constant of protein-protein docked complex?

Hello everyone, I would like to get help about finding any server or application which can give me info about free energy and dissociation constant of protein-protein docking outputs. I have...

15 January 2021 587 3 View

What would be the best option to dock a number of peptides to a protein?

I have been using PyRx to dock peptides to a protein. Initially the numbers were small so it was okay. But now I have to dock over 500 peptides and doing so manually would take forever. My...

10 January 2021 3,330 4 View

Is there any online webserver for DNA-ligand docking?

I have used Autodock 4.2 for docking studies and trying haddock for DNA-Ligand docking. It shows an error and not supported DNA PDB file. Suggest a template for DNA and ligand PDB file and also...

29 December 2020 1,322 8 View

I am looking to learn docking studies online can any one help?

I am interested in docking studies and want to work in that field looking for someone who can help and will guide.

22 December 2020 1,575 2 View