I do not know if a product exists that I can add at the beginning of the immunohistrochemistry process to avoid the different solutions entering under my brain sections and producing this wave effect.
Immunohistochemistry: (S. Renshaw, ed.)
CHAPTER4 Immunochemical staining techniques
http://www.scionpublishing.com/shop/ProductImages/Immunohistochemistry%20Chapter%204.pdf
..."Sections are cut at around 4 μm thickness and floated out in a water bath before being picked up on to a glass microscope slide. The sections are then dried at around 60 °C in order to increase their adherence to the slide and to help to iron out any creases obtained during microtomy or floating. Before immunochemical staining can occur, sections must be dewaxed and any necessary pre-treatments performed.
4 μm thickness tissue floated on 39 to 42degree centigrade water (luke warm). Pick the section gently on clean glease free slide. After picking some times skillfull and experinced person pour 70% alcohol between section and slide so that fold slowly get removed. The sections are then dried at around 60 °C in order to increase their adherence to the slide and to help to iron out any creases obtained during microtomy or floating. then use furthe proceesing procedure.
There is growing evidence - both theoretical and empirical, that implicates travelling waves or solitons (a type of robust standing wave) as the neural correlate of consciousness. There is a small possibility that the wave formations that you are observing might be an artifact of normal brain processing!
One way to test this hypothesis would be to compare adjacent slices to see if the patterns are the same or very similar.
Chapter Life, Catalysis and Excitable Media: A Dynamic Systems Appro...
Christopher, it seems she is talking about rugs in dead tissue
Rather that gelatin, we prefer APES coated slides. Slides should be thoroughfully cleaned and coated with APES. Once dried, 4µm paraffin sections remain completely flat once mounted and dried at 36ºC and so you can perform Immunohistochemistry without any waving effect.
Here you can found the APES coating protocol we use for all kind of paraffin embedded tissues:
http://methodbook.net/probes/insitu.html
You can check image quality in figures 3 and 4:
http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00170/full
Best
Enrike
Hi Patricia,
assuming that your cut sections are flat to begin with, the cause is likely to be partial loss of section adhesion. This is be more prevalent in brain than other tissues and so it might not have seen it in other tissues. The suggestions above for gelatin or APES slides are partially correct but slide technology has moved on since then. Most IHC labs, like my own, use charged slides to avoid what used to be a common problem. Superfrost Plus (or equivalent) slides are produced by a number of manufactureers and sections should be dried at 60 C for 1-2 hours. They are particularly "sticky" and do not result in background staining as can be the case with APES or Gelatin.
Kind regards,
Kieran
- Badges
- Science topic