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Questions related from Patricia Sampedro Piquero
We are interested in using this task in our experiments with Wistar rats. What is the best protocol?
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I do not know if a product exists that I can add at the beginning of the immunohistrochemistry process to avoid the different solutions entering under my brain sections and producing this wave effect.
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The rats (20 months-old) were deeply anesthetized with sodium pentobarbital and transcardially perfused with 4% paraformaldehyde. The brains were included in paraphin and cut in a microtome at 20...
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