we need to remove bacterial lysate debris and culture media constituents while we purify the phage and use it for labelling with any signals like 125-I or FITC. But the phage aggregates during the purification. I have tried CSCl Density gradient, PEG/Ammonium sulphate How can I keep  the phages in solution or as a suspension of monomers while keeping them free of any other added proteins in the solution

When I concentrate the T7-Phages by PEG/Ammonium sulphate and band it out on a CsCl density gradient they aggregate and the phages donot go back in to their monomeric status because they are not in a protenacious environment. where as I need to keep them in a protein free medium in-order to labell them with either FITC or 125-I 

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