The alginate hydrogel was fixed in 4% PFA for 2h, dehydrated in 30% surcose overnight, and embed in OCT. After snap frozen, the alginate hydrogel was cryo-sectioned onto a charged glass slide. However, during HE staining, the samples will detached from the slides. Is there a way to prevent the sections from detachment?
you could try to coat it with something like gelatin perhaps, but I would rather just use Superfrost plus glass slides from either Fisher Scientific or VWR
Hi Wen Xue,
You can try increasing the concentration of PLL used or by coating with gelatin as mentioned before by the researcher. The slides need to be socked in PBS or water to remove OCT and sucrose carefully before proceeding for staining otherwise chances of detachment are more.
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