I need to analyze the gelling consistency of a rice flour sample. According to the method I followed, I need a 0.025% Bromothymol blue ethanol solution. I only have Bromothymol powder. Please assist me with proper steps for making this solution.
Dissolve 25 mg BTB and make up to 100 ml in a volumetric flask with ethanol.
I need to create a few 3D graphs using RSM which is available in Design expert.
29 June 2023 9,621 4 View
We have transfected HEK293 Cells with a viral gene. I have extracted genomic DNA from the mammalian cells. How can I determine the viral gene copy number using qPCR? Thanks in advance for the...
04 January 2023 9,301 4 View
My questionnaire consist of 20 questions and five of them are related to dependent variable. But the problem is those questions are not in Likert scale and in different scales with fixed answers...
13 December 2022 9,655 4 View
I have added the groovy boundary conditions on the temperature at the free surface to calculate the radiative heat transfer. However, when I calculate the case using this boundary condition, the...
06 December 2022 524 0 View
I want to amplify RAPD and ISSR markers using primers which has 27-37 0C of annealing temperatures. I'm conducting this study for a plant evolutionary study. I've tried several times with Solis...
14 October 2022 4,275 1 View
I am working on rainfall run-off predictions using a novel algorithm called the Cascaded ANFIS algorithm. Does anyone know a way to obtain SSP daily rainfall data of a specific geographical area?...
05 July 2022 8,543 1 View
Dear Colleagues, I have conducted a sensory evaluation for 8 biscuit samples using 7 point hedonic scale with 30 semi-trained panelists. The data obtained from the sensory evaluation was analyzed...
10 July 2021 4,990 7 View
I need to download instagram data for a research project. It seems like almost all APIs which provided access to instagram data have been restrained by facebook company.
31 July 2020 1,910 2 View
Hello Just wondering when I stimulate the HL60 cells with DMSO and use them around D7, do you advise to change the medium just like how we normally pass down the cells? Or should I keep them in...
07 October 2019 6,976 2 View
Dear Colleagues, Can anyone describe how Water Absorption Index, Water Solubility Index and Swelling Power of the flour type we use for bakery products can affect the porous crumb structure...
08 June 2019 4,740 2 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
https://youtu.be/5MckkqF9XWU?si=MvBsElJZg8_At8qP
09 July 2024 6,038 5 View
What is safe gasoline and Ethanol safe or Ethanol petrol mixture safe for car fuelin.
03 July 2024 2,939 3 View
My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...
17 June 2024 4,183 3 View
Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...
16 June 2024 2,754 0 View
We are trying to use 100% ethanol as a precipitant for Bovine Serum Albumin. The BSA is in a 0.5% concentration and we are using various ratios of ethanol to attempt to precipitate it, such as 1:2...
04 June 2024 8,024 1 View
I'm trying to identify an organic compound from a 48-hour-old bacterial broth culture. To do this, I need to remove the sugar and then purify the sample using C18 cartridges. However, the C18...
21 May 2024 8,722 0 View
Hi I have been working on droplets, lipid vesicles for my experiment and I have a serious issue that my droplets get stuck on the coverslip. I use the following protocol below to make my...
21 May 2024 9,285 3 View
and after sintering
12 May 2024 5,031 3 View