I am interested to determine pesticide derogation using microorganism. For this reason soil extract liquid broth necessary. Its too difficult to prepare using conventional method.
Hi Mohammed,
it depends on whether you want to use the medium to study degradation or to isolate the pesticide degrading organisms. To study degradation I would suspend soil in non-sterile tap water mix it well and then collect the liquor through a charcoal free filter paper. This would serve as a growth medium one the pesticide of your choice was added. However it would contain a complex community of microorganisms so detection of a single organism may be quite tricky. Avoid autoclavation as it changes the chemistry of the soil extract (particularly Calcium and Magnesium).
If you intend to use the medium for growth of an isolate in pure culture then I suggest you make the broth as above and then centrifuge and filter though 0.22um filter. This should give a sterile medium you can use.
Of course the exact content of your broth will be unknown (a complex medium) and will be dependent upon the soil sample you used in the first place.
For growth studies on a chosen pesticide 0.03% yeast extract will help to start your organisms growth off.
hope this helps
cheers,
Richard
Hi Mohammed,
it depends on whether you want to use the medium to study degradation or to isolate the pesticide degrading organisms. To study degradation I would suspend soil in non-sterile tap water mix it well and then collect the liquor through a charcoal free filter paper. This would serve as a growth medium one the pesticide of your choice was added. However it would contain a complex community of microorganisms so detection of a single organism may be quite tricky. Avoid autoclavation as it changes the chemistry of the soil extract (particularly Calcium and Magnesium).
If you intend to use the medium for growth of an isolate in pure culture then I suggest you make the broth as above and then centrifuge and filter though 0.22um filter. This should give a sterile medium you can use.
Of course the exact content of your broth will be unknown (a complex medium) and will be dependent upon the soil sample you used in the first place.
For growth studies on a chosen pesticide 0.03% yeast extract will help to start your organisms growth off.
hope this helps
cheers,
Richard
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