Dear Krishna,

you may try this method published by the Gene expression Center of University of Wisconsin

https://www.genome.wisc.edu/pub/reprints/GEC_Slide_Coating.pdf

The University of Wisconsin, Gene Expression Center

Genetics/Biotechnology Center, Genetics Bldg. Room 302

445 Henry Mall

Madison, WI 53716

Slide Coating (polylysine treatment)

Date

During all procedures wear powder free gloves and wash fresh gloves with soap and water.

This protocol is for coating 60 slides.

1.

Load 60 slides (GoldSeal) into stainless steel slide racks (30 slides/rack). Check each slide

for scratches and defects. Discard any questionable slides.

2.

Prepare cleaning solution as follows: 350ml/slide rack

For 350ml

For 700ml

50g NaOH

100g NaOH

Lot#

100ml MQ H

2

O

200ml MQ H

2

O

NaOH (g)

250ml 95% EtOH

500ml EtOH

Dissolve NaOH in water and slowly add EtOH

(Caution EXOTHERMIC Rx)

Add additional MQ H

2

O until solution is clear (about 100ml/350ml solution)

3.

Add 350ml of cleaning solution to each glass dish with slides. Cover dishes with glass and

shake gently for 2 hrs.

4.

Remove slides from cleaning solution and plunge into 400ml fresh MQ water in dish. Plunge

up and down vigorously and shake for 5 minutes.

5.

Repeat this wash step 5 times with fresh MQ water for each wash. It is imperative that all

NaOH is removed before polylysine coating.

X X X X X

6.

Prepare polylysine solution

For 350ml

For

700ml

35ml polylysine (Sigma)

70ml polylysine

Lot#

35ml 1X PBS (filtered)

70ml 1X PBS

Expiration Date

280ml MQ H

2

O

560ml MQ H

2

O

7.

Add 350ml of polylysine solution to a clean glass dish and submerge washed slides (in rack)

and shake gently for 1hr.

8.

Remove slides from polylysine and rinse by plunging 10X into fresh MQ H

2

O.

9.

Spin slides in slide rack at 500 RPM, 5 minutes, wrap slides in aluminum foil and place

slides in 42

o

C oven for 2-3 hrs.

Note. At this point you want to minimize exposure of slides to dust. Working in a hood, transfer

slides to clean slide box and label box with the date that the slides were coated and name of

person performing coating.

**Slides must be aged at least 2 weeks prior to printing. The aging allows slides to become

sufficiently hydrophobic so that printed DNA is maintained in compact spot.

The University of Wisconsin, Gene Expression Center

Genetics/Biotechnology Center, Genetics Bldg. Room 302

445 Henry Mall

Madison, WI 53716

If you have less time to prepare your slides, I recommand Superfrost Plus slides. I have made very good expiriences especially when I react 20 µm thick sections.