How can I prepare a protein sample for atomic absorption spectroscopy?

More Chinmoy Banerjee's questions See All

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

What is the reason for the non-monotonic variation of roughness for sputtered Cu films annealed at 100, 200 and 300 degree Celsius?

I have prepared Copper thin films by magnetron sputtering and the samples have been annealed at 100, 200 and 300 degree celsius. The variation of the rms roughness and all other AFM height related...

11 June 2024 9,774 1 View

I see GFP signal on cells even though there is no GFP in them. Can anyone tell me what could be the reason?

These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...

06 June 2024 2,289 3 View

Can anyone help me with GCF (Gingival Crevicular Fluid) elution from PerioPaper?

I am using PerioPpaer from Oraflow, to collect GCF, 8-12 PerioPapers from each subject and a pooled sample in PBS elution buffer. For the elution, I am vortexing for 1 min, then slow...

03 June 2024 9,767 0 View

How to cal culate the direction of dipolemonet for a molecule energy optimized using Gaussian package?

I performed single-point energy optimized using Gaussian software, followed by mapping the electrostatic potential surface around that. There is a clear dipole for that molecule but I want to...

24 May 2024 8,549 1 View

Could anyone kindly provide me the data regarding the variation of water flow rate for 24 hour horizon for small hydro power used in multi period OPF?

Optimal power flow, small hydro

20 May 2024 7,590 0 View

How to calculate the angular velocity of a rotating cell from a microbe video?

I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...

18 May 2024 4,289 3 View

I have docked 17 antiviral drugs using UCSF Chimera AutoDock Vina. I need to select the best 3. How do I do that?

I need to select 3 best results from the list of the 17 antiviral drugs. How do I select that?

25 April 2024 5,386 1 View

Bone material: What this minimum of maximum strain of Human cortical Bone before fracture?

What this minimum of maximum strain of Human cortical Bone before fracture. I saw lot of papers showing different values.

27 March 2024 4,255 1 View

What type of seagrasses and saltmarsh grasses are found in coastal odisha?

explain its morphology, taxonomy and habitat distributiion

03 March 2024 1,293 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

Vandana Singh

AAS is used to detect heavy metal while flame photometer is used to detect calcium (alkaline earth metals). you can detect Ca in yr sample by using bi acidic digestion method followed by analysis through flame photometer

Lukasz Wojciech Wajda

Add 10 ml of concentrated nitric acid of high purity, digest your samples in microwave oven and dilute further if necessary with deionised water. Then use FAAS to determine calcium in your samples.