How can I performance ChIP(chromatin immunoprecipitation) with Arabidopsis pollen?

24 February 2019 1 9K Report

I am trying to perform ChIP with Arabidopsis pollen, but the literature I could found only mentioned ChIP with seedlings or inflorescences or detection of histone variants and modifications.

Are there any existing protocols or someone could tell me how to try to develop a system and test it, thanks.

Hua Xiao

Dear Changdi, I have never done ChIP with Arabidopsispollen, but I think you can collect its pollen and then follow the other standard protocols starting with cell lysis/fixing. Hope this helps.

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