Every cytokine has its own signaling pathway and don't effect other cytokines expression?

Is it correct to say that IL-6 has a seperate signaling pathway and don't effect expression of TNF -a in cell culture model ? Any answer appriciated.

06 March 2019 9,169 38 View

Can IL6 upregulate TNF-a and IL-8 in in vitro cell culture model?

Hi, I am working with an inflammatory cell culture model which the stimulater is IL6 10 ng /ml . But after checking the pro-inflammatory cytokines like TNF-a ,IL8, INF-a , non of them has been...

28 February 2019 4,232 4 View

What is the best way to calculate the ELISA result?

I have used excel and Prism to analyse the results. I have some sample values which are below blank (OD). If I substract the OD value of these samples from blank I do get VERY LOW or negative...

02 February 2019 7,112 6 View

What is the best assay to confirm the result from ELISA?

Hi, I am checking the antiinflammatory effect of few substances on a co-culture model. I have screened the pro-inflammatory cytokines with ELISA kits. Do I need to confirm the result with...

22 January 2019 4,845 7 View

Can ELISA on cell lysate give more detectable results for cytokines compare to basolateral medium?

Can ELISA on cell lysate give better and detectable results for cytokines compare to basolateral medium?

08 January 2019 6,692 3 View

Does phenol red interfer with ELISA?

Hi, I am going to run an ELISA- a colorimetric based method. I have phenol red in my samples. I read both negative and nutral impact of phenol red on ELISA. I want to know if really phenol red...

06 December 2018 3,191 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

How the precipitation Ratio and flow Ratio effect the output of the result?

I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?

22 January 2021 152 2 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

HEC RAS: project file error?

When I try to create a new project file on HEC RAS, this message appears "title and file name must be specified", even when I have already specified them.

03 January 2021 5,618 2 View

How to download future precipitation data for years 2021-2030?

For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?

21 December 2020 8,928 5 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View