How can I measure the ROS of bacteria using -H2DCFD? I am confused by reading different methods in papers and no one wrote the method with details . Is there any protocol for detecting ROS in bacteria in liquid media?
I recommend that after you place the indicator to ROS, protect your samples from the light (15 MINUTES). Paper: https://www.frontiersin.org/articles/10.3389/fmicb.2019.02928/full