OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

Interpreting regression findings prior to inclusion of control variable?

I'm looking for advice on interpreting a statistical relationship - it's for a paper I am reviewing. (Variable names have been changed). It was found that: [IV anxiety + control variables y and...

06 January 2021 558 3 View

Can someone help me with an extraction protocol?

Hi all, Does anyone have a protocol for extracting DNA from agarose that is only 120 bp without a kit? The protocols I have tried are for much larger fragments and it is just not working. Thank you!

10 September 2020 1,291 2 View

Advice for getting dermatopathology tissue to stick to slides during immunostaining?

I work in a veterinary pathology lab and we do immunohistochemistry staining by hand on all types of tissues that are FFPE. We have particular difficulty getting dermatopathology (skin) samples to...

26 August 2020 792 1 View

Validation of experimental results?

Is it neccessary to perfrom validation of process model established using a box-benkhen experimental design? How "big mistake" will it be if I finish the experiment after performing the runs...

24 August 2020 6,917 3 View

Any reliable protocols for tissue detection of DNA damage with IHC or IF?

Dear all, Hope as a community your are doing fine. I have a question regarding antibodies against gH2AX, XRCC1 and p53bp1 that have worked for tissue detection for either IHC or IF, using...

08 June 2020 1,461 3 View

Where can I find the parameters of Miltenyi gentleMACS protocols?

I'm planning to use a Miltenyi brain tumour dissociation kit, but without the gentleMACS machine. Does anyone know where I can find the parameters of the machine's programs/protocols?...

16 December 2019 6,150 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

What is the best statistical method for analysis of pooled fecal samples?

Hello We performed a study on the intestinal microbiota of rats in six intervention groups (8 rats in each group). It is suggested that since the rats in each group were the same in terms of...

10 January 2021 2,507 4 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Fish gut microbiota extraction and identification protocol?

Hi, I need a protocol to extract and identify microbiota from fish gut. Any suggestions? Thank you. (P.s. Something low tech as I am technologically limited, guess no funds and big high tech...

01 January 2021 808 1 View

How to functionalize mineral surfaces with silanes?

I would like a method to modify mineral surfaces using silanes. The purpose is to alter the hydrophobicity of the minerals so as to test them in a flotation exercise. Is there a way to effectively...

29 November 2020 8,243 7 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

25 November 2020 2,680 3 View

What is singletons in microbiota data?

I have seen in papers that many papers remove singletons from their microbiota data. I am not sure what is actually singletons and why the authors remove the singletons sequences. I would be happy...

23 November 2020 4,649 6 View

What can we infer from different microbial communities in fish gut from two different locations?

Recently I have been interested in microbial communities from gut & intestine of the same fish species popular in Indonesia marine aquaculture, Grouper (Epinephelus sp.), but are raised in...

18 November 2020 510 5 View

Does Arsenophonus replace the other gut bacteria in insects?

02 November 2020 4,628 4 View

Can anyone tell why SF11 glass turns black sometimes during cleaning with Piranha solution?

I have experienced that SF11 glass (coated with some metal) turns transparent black/grey once metal gets removed with piranha solution. Please state the reason and prevention. Thanks in advance :)

20 October 2020 5,129 3 View