Hi,

I would not directly measure PCR products using a nanodrop since there is too much variation coming from the component of the PCR solution. Thus, the result is most likely an unreliable mesurement of the amount of DNA in the sample. 

If you want to use nanodrop, you need to purify your PCR product and then use a nanodrop to estimate the concentration of your PCR products. However, this method is tedious, just to obtain a semi-quantitative result.

Thus, I would favor a standard agarose gel electrophoresis approach (especially if your master mix is colored and I guess loading ready) or the usage of conventional qPCR detection methods.

Good luck,

Nicolas

Thank you Nicolas. Actually this exactly what I did before bu I just wanted to see if there is any faster and cheep method to perform. Anyway, I appreciate your help.

Best regards

A better way to measure DNA post-PCR that  I would recommend is Quantitative gel electrophoresis  using  known concentrations of DNA standards instead o using nanodrop. 

Thanks Amal

I agree with you but it is time consuming method and logically can not considered when the number of samples is really high.

Regards