As there are many different size of cells (myoblast, myotube,nascent myotube) on my image, and I would like to measure/analysis only on myotubes which it has width size (0-60 micron) and length (0-800 micron). The problem is I do not know how to adjust threshold only on myotube images, and it always have noise/unselected area appreded when I chose analyze particle. Any comments are welcome.
Valeria De Arcangelis
http://rsbweb.nih.gov/ij/docs/examples/index.html, only imageJ homepage to known how to use better this tool This is very useful fo me: Part3: http://serc.carleton.edu/eet/measure_sat2/part_3.html for width (or diameter), I suggest you to click on the example, and then to part4 for the area (on the left column).In general I only measured myotube width and area not lenght because when you take images it is difficult to have in one photos the entire myotube, but you can find a lot of literature that can help you how to make your measure you can find tutorial on you tube https://www.google.it/search?q=lenght+myotube+tutorial&ie=utf-8&oe=utf-8&aq=t&rls=org.mozilla:it:official&client=firefox-a&channel=nts&gfe_rd=cr&ei=V6gyVLGVEcTo-gaBz4DIAg#q=length+myotube+image+J&rls=org.mozilla:it:official&channel=nts&start=10
Hi Pornthida, you can use this links:
Good measure!!!
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Pornthida Poosala
Hi Valeria, thank you for your suggestion and comments.
Hi Mohamed, I can't upload pics, because it is unpublished data.
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