Quick way to collect saliva to study viral RNA expression?

Hello, I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and...

24 September 2020 4,937 4 View

What have been the effects of Covid-19 on digital engagement in the Heritage industry?

For my dissertation i am looking at digital history and digital engagement and how this has been effected by the Covid-19 pandemic. Any help with this research would be greatly appreciated.

29 July 2020 2,808 3 View

Why my PCR amplicons no longer have the expected size and are faint?

Dear all, I have issues with my PCR amplicons. For one week now, I do no longer have the expected size for my PCR amplicon (252 bp in the first 4 wells) on the 50 bp DNA ladder. They appear faint...

17 July 2020 4,988 5 View

Which is the best substrate/growing medium to apply controlled drought to corn?

Hi, I'd like to apply a controlled water stress on corn at V5 stage in order to verify the effects of a biostimulant in responding to the stress. It would be a dry down experiment and I'll apply...

07 March 2019 5,293 7 View

Any NK cell line that express inhibitory killer cell immunoglobulins-like receptors (iKIRs)?

I wish to investigate the influence of iKIRS in natural killer cells cytotoxicity to the tumor cells, yet i dont seem to get any NK cell line that expresses iKIRS.

21 May 2018 3,100 1 View

Why my ligation presents non specific bands?

I'm tring to insert a fragment with 342pb in the pENTR/SD/D TOPO cloning vector, that have about 346pb. I have already purified the gel with the DNA fragment and did the ligation, using the...

17 January 2018 9,882 4 View

No title

19 April 2017 4,073 3 View

I want to preview BRATs medical image using MATLAB

I used the code "Read 3D data " in Matlab to read in my medical image but i cant view the image yet i guess it has to do with rescaling. this is the message in the command window. RescaleSlope not...

01 January 1970 6,083 5 View

Police leaving the force to join private investigations

Hi Everyone, I am looking for officers who have quit the force and joined the PI field for what ever reason. Last year it was reported that there was a shortage of officers because many quit the...

01 January 1970 1,813 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View