How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Why high infiltration in OM treated plot?

We are carrying out trial in the glasshouse where we are comparing the infiltration rate in sodic soil in two different treatments Polyacrylamide vs organic matter ( lucerne pellet). within 27...

25 November 2020 8,787 3 View

I am having problems in removing m-cpba and its corresponding acid ?

I am doing alkane oxidation using m-CPBA as oxidant in DCM. How can I remove the oxidant and its corresponding acid (m-CBA) from my reaction mixture? I also did aqueous work-up using NaHCO3 and...

13 August 2020 8,290 3 View

What are the alternative way to identify the stem cells present in cord blood??

commonly used practices for the identification and characterization of the kind of cell in cord blood other than Flowcytometry and reverse-transcription polymerase chain reaction (RT-PCR).

08 August 2020 6,881 3 View

Why Nepal's agriculture sector growth is staggering?

Agriculture is the major contributor to the national economy, livelihood and employment since long. However, despite the priority in the national policies, the growth of the agriculture sector is...

05 June 2020 1,079 2 View

VCF File SNP Analysis ??

Hello all, I have 60 samples of Whole Genome Sequence (BAM, VCF ) File. Now I want to do SNP analysis ( I am trying to find some positions which differ among case and Control ). I did with...

30 May 2020 980 4 View

Does the internet based survey research need ethical approval?

As internet is, now-a-days, a useful tool for data collection and research, issues also go hand in hand with ethical approval and informed consent. As participants are, by default, give their...

25 May 2020 4,055 13 View

What is the appropriate statistical analysis for Pre-test Post test study with control group and binary outcome?

Let us consider, I collected baseline data for control and experimental group. After that i give intervention to experimental group. Later, I collected data from both group after intervention. If...

18 May 2020 6,007 7 View

Is it possible that an anticancer drug IC50 value for a particular cancerous cell line decreases on conjugation with an antibody??

Is it possible that an anticancer drug IC50 value for a particular cancerous cell line decreases on conjugation with an antibody??

22 April 2020 9,482 4 View

Does the poly crystalline tungsten nano tip's atom migrate from one layer to another layer under high voltage in Field ion microscope (FIM)?

In Field ion microscopy (FIM), polycrystalline tungsten nanotip is (110) oriented tip. under the gas etching process, the nanotip becomes more sharp by reducing the atomic layer. During the...

14 January 2020 8,055 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View