hello, i want to study on germination ecology of weeds at different level of pH. proposed research conducted in laboratory and i want to maintain pH in petri dishes.
You can provide solution (with a known pH)to seeds continuously like drip.
There are buffers available in the market. Buffers by design maintain a constant pH.
Bruce Robin Nyamweha if i add just acid or base in the solution after few hours its change pH it may be 5 to 6 or 5 to 4 i want to maintain ph at 5,6,7,8,9,10 i want to make it constant at specific pH ...... i want ask what type of buffers that i can i add in the solution
You can employ Good's buffer, zwitterionic compounds that are often used in biological applications (such as MES, HEPES, MOPS...). They have a good buffering capacity, low toxicity and are not metabolised quickly like common buffers. You need to study some theory. I can recommend this for further reading: https://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf and the attached review.
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