How can we detect over expression of a gene?

Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?

06 July 2019 9,406 5 View

How many bands will I find if I run human extracted DNA through agarose gel electrophoresis?

Human bears separate 46 chromosomes. If we extract DNA from human sample then how many band will be visible on gel?

08 September 2018 6,350 5 View

Do pH maintaining chemicals interfere in DNA extraction?

We use EDTA, Tris-HCl and some other chemicals, whose pH remain almost 8.0. To balance pH 8.0 we use NaOH. Now I would like to know that; have these unwanted NaOH, HCl or Acetic Acid (if...

04 May 2018 5,684 1 View

Which is the solution used for pH meter sensor?

I need to know that which solution is used to keep the pH meter sensor wet. Some people say that KCl is used but which concentration and pH are better?

31 December 2017 5,670 3 View

How can we measure the band size that greater than 10Kb?

07 August 2017 3,413 2 View

Why do bacteria lose their plamids at normal condition?

Plasmids are extra chromosomal DNA of bacteria, but they have numerous functions for the survival and living of bacteria. It is claimed that bacteria lose their plasmids when they live without...

07 August 2017 3,918 9 View

Is it mandatory to culture Bacteria in an antibiotic rich medium before plasmid extraction?

It is claimed that bacteria lose their plasmids unless they are cultured in a antibiotic maintained medium. As a result a negative consequence will arise after plasmid DNA extraction. How will you...

07 August 2017 1,637 6 View

Where are the nod and nif genes of Rhizobia located?

07 August 2017 9,745 2 View

Is it possible to culture coffee plant in vitro?

Is anywhere it being tissue culture of Coffee plant in Bangladesh?

04 May 2017 4,613 2 View

What is selfish Rhizobia?

Somebody claims that there some selfish rhizobia which take benefits from the host but do not induced nodulation at the host. I did not get enough evidence about this. Could anybody help me by...

07 August 2016 3,089 6 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

Sound transmission loss (Acoustic Metamaterial)?

My design contains three parts. A silicon rubber membrane is placed between upper and lower part. I want to calculate sound transmission loss by using comsol Multiphysic. There are lots of...

05 February 2021 466 4 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View