Isolation for mono- as well as sesquiterpenoids the classic procedure is obtaining essential oils by steam distillation. However extraction with non-polar solvents such as petroleum ether, ether and hexane can be preferred due to artifact formation at the raised temperatures. Adsorbtion chromatography on silica gel is the simplest and most effective method for separation of terpenoids and GLC is used commonly for identification as well as isolation of the monoterpenoids. Column chromatography is also a valid method for fractionation of monoterpenoids. Isocratic elutions with solvents such as pentane, petroleum ether, hexane or gradient elution with mixtures of solvents in increasing polarity leads to successive isolation. Additionally, faster techniques of column chromatography such as flash chromatography may be preferred due to conventional column chromatography for separating procedure is time-consuming and frequently gives poor recovery owing to band tailing .
Difficult task. Have a technique using headspace coupled to GC-MS. However, the amount isolated is very small. I by improving the procedure to increase yield. Wait 2015.
You may use preparative GC but you need to have a splitter of the column effluent into FID and for collection. Alternatively, you may use fractional micro-distillation techniques such as Kugelhor
Difficult task. Have a technique using headspace coupled to GC-MS. However, the amount isolated is very small. I by improving the procedure to increase yield. Wait 2015.
Dear Dr. Prabhu
Fractional distillation technique has been widely used for separating major and minor components of essential oils both at laboratory and industrial levels.
You may follow following links for more information:
https://www.researchgate.net/post/Hos_do_I_isolate_the_major_components_of_essential_oils
https://www.researchgate.net/post/How_can_I_purify_individual_components_from_essential_oils
Best wishes and good luck.
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