Please have a look at the enclosed PDF

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Please take a look on pending PDF,s

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I think you can follow Dr Srivatava comment

After uprooting, about 15 nodules (≥ 2 mm) were collected per pot from Yezin-6 (non-Rj) and Yezin-3 (Rj4), respectivley. A total of 30 nodules from each sampling site were used to extract bacteria. Nodules were arranged from the largest to the smallest size to designate the name of isolates numerically. For surface sterilization, the nodules were immersed in 70% ethanol for 3 min, soaked in 2.5% sodium hypochlorite (NaClO) solution for 15 min, and washed five times with 0.9% autoclaved sodium chloride (NaCl) solution. The surface-sterilized nodules were transferred separately into the autoclaved small test tubes and crushed. The milky bacterial juice was streaked onto Yeast Extract Mannitol Agar (YMA) plates (Vincent, 1970) containing 25-µg Congo red (Somasegaran and Hoben 1984), using sterilized glass rods. The plates were incubated at 30°C for 7 days to promote colony development.

follow the methods of  (Vincent, 1970) and (Somasegaran and Hoben 1984)

Thank you sir